Supplemental Data A Slowed Classical Pathway Rather Than Kiss-and-Run Mediates Endocytosis at Synapses Lacking Synaptojanin and Endophilin
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چکیده
(1993). Targeted gene expression as a means of altering cell fates and generating dominant phenotypes. Development 118, 401-415. distribution of the cysteine string proteins in Drosophila tissues revealed by targeted mutagenesis. (1996). Traffic of dynamin within individual Drosophila synaptic boutons relative to compartment-specific markers. (2003). Endophilin promotes a late step in endocytosis at glial invaginations in Drosophila photoreceptor terminals.
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Matters Arising A Slowed Classical Pathway Rather Than Kiss-and-Run Mediates Endocytosis at Synapses Lacking Synaptojanin and Endophilin
The extent to which a “kiss-and-run” mode of endocytosis contributes to synaptic-vesicle recycling remains controversial. The only genetic evidence for kiss-and-run at the synapse comes from mutations in the genes encoding synaptojanin and endophilin, proteins that together function to uncoat vesicles in classical clathrin-mediated endocytosis. Here we have characterized the endocytosis that pe...
متن کاملA Slowed Classical Pathway Rather Than Kiss-and-Run Mediates Endocytosis at Synapses Lacking Synaptojanin and Endophilin
The extent to which a "kiss-and-run" mode of endocytosis contributes to synaptic-vesicle recycling remains controversial. The only genetic evidence for kiss-and-run at the synapse comes from mutations in the genes encoding synaptojanin and endophilin, proteins that together function to uncoat vesicles in classical clathrin-mediated endocytosis. Here we have characterized the endocytosis that pe...
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We describe the isolation and characterization of Drosophila synaptojanin (synj) mutants. synj encodes a phosphatidylinositol phosphatase involved in clathrin-mediated endocytosis. We show that Synj is specifically localized to presynaptic terminals and is associated with synaptic vesicles. The electrophysiological and ultrastructural defects observed in synj mutants are strikingly similar to t...
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Endophilin is a membrane-associated protein required for endocytosis of synaptic vesicles. Two models have been proposed for endophilin: that it alters lipid composition in order to shape membranes during endocytosis, or that it binds the polyphosphoinositide phosphatase synaptojanin and recruits this phosphatase to membranes. In this study, we demonstrate that the unc-57 gene encodes the Caeno...
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Synaptojanin and endophilin represent a classic pair of endocytic proteins that exhibit coordinated action during rapid synaptic vesicle endocytosis. Current models suggest that synaptojanin activity is tightly associated with endophilin through high-affinity binding between the synaptojanin proline-rich domain (PRD) and the endophilin SH3 domain. Surprisingly, we find that truncated synaptojan...
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